Cloning and Expression in E. coli and Enterococcus

All cloning was done in chemically competent E. coli Dh5α (New England Biolabs, NEB) according to the manufacturer’s protocol (pAM401 plasmids = 25 μg mL^–1 chloramphenicol; pET-21a(+) plasmids = 100 μg mL^–1 ampicillin). Colonies were picked and verified by Sanger sequencing (Genewiz). Confirmed pAM401 plasmids were transformed into electrocompetent Efm Com15 (Table S1) according to a Palmer laboratory Enterococci transformation protocol^{14 (link)} for constitutive expression (10 μg mL^–1 chloramphenicol). Confirmed pET-21a(+) plasmids were transformed into competent E. coli BL21-CodonPlus (DE3)-RIL (Agilent) according to the manufacturer’s protocol for IPTG inducible expression (100 μg mL^–1 ampicillin and 25 μg mL^–1 chloramphenicol; see note in Table S1).