5637, T24 and SW780 cell apoptosis was analyzed using an Annexin V-FITC/PI double staining kit (Invitrogen, Carlsbad, Calif, USA) with flow cytometry (FACScan, BD Biosciences, San Jose, CA, USA) as described previously [20 (link)]. Apoptotic cell proportion was calculated using CELL Quest 3.0 software (BD Biosciences).
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Annexin V-FITC/PI Apoptosis Analysis
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Corresponding Organization :
Other organizations : Xiangya Hospital Central South University, Central South University, Hunan Provincial People's Hospital, Fourth People’s Hospital of Jinan, Affiliated Hospital of Hebei University
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Variable analysis
independent variables
- Cell lines (5637, T24, and SW780)
- Apoptotic cell proportion
- Annexin V-FITC/PI double staining kit (Invitrogen, Carlsbad, Calif, USA)
- Flow cytometry (FACScan, BD Biosciences, San Jose, CA, USA)
- CELL Quest 3.0 software (BD Biosciences)
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