Neutralization Assay of Inactivated Sera

Neutralization of irradiated and heat-inactivated serum samples were assessed in Vero UNC cells. Neutralization assays with VSV-TAFV-GFP were performed as previously described [16 (link)]. Samples were run on the FACSymphony A5 Cell Analyzer (BD Biosciences, Mississauga, ON, Canada) where the GFP-positive cell count was measured and data analysed on FlowJo V10.

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Fletcher P., O’Donnell K.L., Doratt B.M., Malherbe D.C., Clancy C.S., Rhoderick J.F., Feldmann F., Hanley P.W., Ksiazek T.G., Geisbert T.W., Messaoudi I, & Marzi A. (2023). Single-dose VSV-based vaccine protects cynomolgus macaques from disease after Taï Forest virus infection. Emerging Microbes & Infections, 12(2), 2239950.

Publication 2023

FACSymphony A5 Cell Analyzer

Assays Cell Serum Vero cells

Corresponding Organization : National Institutes of Health

Other organizations : University of Kentucky, The University of Texas Medical Branch at Galveston

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Variable analysis

independent variables

Irradiated and heat-inactivated serum samples

dependent variables

Neutralization of serum samples in Vero UNC cells
GFP-positive cell count measured by FACSymphony A5 Cell Analyzer

control variables

Neutralization assay protocol previously described [16]

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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