Molecular Tools for Neuroscience Research

pCA-EGFP and pAAV-CAG-TdTomato were previously described [20 (link), 21 (link)]. pAcGFP1-Golgi was obtained from Clontech. For construction of pCAGGS-GRASP65-GFP, cDNA encoding GRASP65 was cloned by PCR from a cDNA library. The PCR product was subcloned into pEGFP-N1 vector (Clontech), and the EGFP-fused GRASP65 was subcloned into pCAGGS vector. Antibodies used for immunostaining were as follows: rabbit anti-Math2, anti-Prox1 and anti-Synapsin I, chick anti-NeuN (Abcam); mouse anti-GM130 (BD Biosciences); rabbit anti-Calbindin, anti-GFAP and mouse anti-GAD67 (Chemicon); mouse anti-PSD95 (Funakoshi); mouse anti-Prox1 (Millipore); goat anti-Ankyrin G (Santa Cruz); rabbit anti-Calretinin (Swant); Alexa405-, Alexa488-, Alexa568-, Alexa633- or Alexa647-conjugated anti-chick, anti-goat, anti-mouse or anti-rabbit IgG (Molecular Probes).