Formalin fixed E18.5 lungs were paraffin embedded and sectioned according to standard procedures. Paraffin sections were stained using antibodies specific to FOXF1 (Malin et al., 2007 (link)), FLK1 (Santa Cruz Biotechnology, Dallas, TX, USA), proSPC (Ustiyan et al., 2012 (link)) and α-SMA (Abcam) as described (Ustiyan et al., 2009 (link); Wang et al., 2010 (link)).
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