Lifespan Assay for Worms with FUdR

L4 -stage worms synchronized from eggs were placed on NGM plates containing 100 μM 5-fluoro-2′-deoxyuridine (FUdR; F0503, Sigma-Aldrich) to prevent progeny production. The number of worms that were alive or dead was monitored and scored at regular intervals of 1 or 2 days. Worms that were ruptured, burrowed, or crawled off the plates were censored but included in the life-span analysis as censored animals. The mean life span and life span assays were analyzed using online application for the survival analysis (OASIS; http://sbi.postech.ac.kr/oasis/surv/)^{69 (link)}.

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Bae E.J., Kim D.K., Kim C., Mante M., Adame A., Rockenstein E., Ulusoy A., Klinkenberg M., Jeong G.R., Bae J.R., Lee C., Lee H.J., Lee B.D., Di Monte D.A., Masliah E, & Lee S.J. (2018). LRRK2 kinase regulates α-synuclein propagation via RAB35 phosphorylation. Nature Communications, 9, 3465.

Publication 2018

F0503

Animals Assays Deoxyuridine Eggs Oasis Worms

Corresponding Organization :

Other organizations : Seoul National University, National Institute on Aging, National Institutes of Health, University of California, San Diego, German Center for Neurodegenerative Diseases, Kyung Hee University, Konkuk University

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Variable analysis

independent variables

5-fluoro-2′-deoxyuridine (FUdR)

dependent variables

Number of worms that were alive or dead
Mean life span

control variables

L4 -stage worms synchronized from eggs
NGM plates

controls

Positive control: Not mentioned
Negative control: Not mentioned

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