Quantitative Methylation Analysis of ALDH2 Promoter

The Sequenom MassARRAY platform was used for the quantitative methylation analysis of the upstream sequence of ALDH2 gene promoter. The methylation status of a detected pattern was then analyzed using Epityper software version 1.0 (Sequenom, San Diego, CA, USA). The promoter regions of the upstream sequence were chosen according to the website: http://genome.ucsc.edu. PCR primers used in this system (Table 2) were designed using predict software (Methyl Primer Express v1.0.exe).
The procedures and reaction system were reported before [15 (link)]. The region analyzed and the CpG sites of the upstream sequence are shown in Figure 2 and Table 3. The same experiments were repeated in triplicate. The methylation level was presented as the ratio of methylated cytosines over the total number of methylated and unmethylated cytosines.