The organic matter (%) was quantified through the loss-on-ignition method by following Wu et al. [39] (link). This method involves preparing the sample, extracting organic matter, and quantifying it through techniques such as gravimetric analysis or spectroscopy. The content of lignocellulose in each treatment was estimated. For that, hemicellulose in soil was determined by separate determination of neutral detergent fibers (NDF) and acid detergent fibers (ADF) [40] (link). Lignin was determined as Klason lignin using 72% (v/v) sulfuric acid [41] (link), while cellulose content was measured at 620 nm using anthrone reagent [42] (link).
Protocol detail
Quantifying Organic Matter and Lignocellulose
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Corresponding Organization : Southwest University of Science and Technology
Other organizations : South China Agricultural University, Guangdong Ocean University
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Variable analysis
independent variables
- Treatment (e.g., different soil types or conditions)
- Organic matter (%)
- Hemicellulose content
- Lignin content
- Cellulose content
- Preparation method for the sample (as described in Wu et al. [39])
- Extraction and quantification techniques for organic matter, hemicellulose, lignin, and cellulose (as described in the respective references)
- No positive or negative controls were explicitly mentioned in the information provided.
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