Genetic Manipulation of Murine Immune Cells

Sox4^fl/fl, Tcf7⁻/⁻, and Cd247^6f/6f (all C57BL/6 background) mice were described previously (Hwang et al., 2012 (link); Malhotra et al., 2013 (link)). Rorc-Cre and Rag1^−/− mice were purchased from Jackson Laboratories. Sox13 promoter–ECFP “knock-in” mice were generated in house, and its characterization is included in Spidale et al. (2018) . Mice were generally analyzed at 4–6 wk for thymic developmental studies and 8–12 wk for peripheral iNKT cell persistence and function. Mice were always age and sex matched within an experiment. All experiments were approved by the University of Massachusetts Medical School Institutional Animal Care and Use Committee.