A scratch wound was created using a Culture-Insert (BD Labware Europe, Le Pont De Claix, France) following the procedures described in our previous study [17 (link)]. The wound was photographed at 0 and 12 h post-infection, and the cells that migrated into the cleared wound area were counted using ImageJ (http://imagej.nih.gov/ij/download.html) and the reduction of the wound area was measured.
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