PBMC Isolation and T Cell Purification

This was performed as previously described.^{35 (link)} In brief, peripheral blood was drawn into EDTA-coated tubes. PBMCs were isolated through centrifugation with Ficoll-Paque (GE Healthcare, Burnaby, BC, Canada), and T cells were isolated from the PBMCs using CD3+ Dynabeads (Life Technologies, Burlington, ON, Canada).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Nieratschker V., Massart R., Gilles M., Luoni A., Suderman M.J., Krumm B., Meier S., Witt S.H., Nöthen M.M., Suomi S.J., Peus V., Scharnholz B., Dukal H., Hohmeyer C., Wolf I.A., Cirulli F., Gass P., Sütterlin M.W., Filsinger B., Laucht M., Riva M.A., Rietschel M., Deuschle M, & Szyf M. (2014). MORC1 exhibits cross-species differential methylation in association with early life stress as well as genome-wide association with MDD. Translational Psychiatry, 4(8), e429-.

Publication 2014

Ficoll-Paque CD3+ Dynabeads

Blood Centrifugation Edta Ficoll T cells

Corresponding Organization : Central Institute of Mental Health

Other organizations : McGill University, University of Milan, Life & Brain (Germany), University of Bonn, National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Istituto Superiore di Sanità, University Medical Centre Mannheim, St. Marien- und St. Annastiftskrankenhaus

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Not explicitly mentioned

control variables

Peripheral blood was drawn into EDTA-coated tubes
PBMCs were isolated through centrifugation with Ficoll-Paque (GE Healthcare, Burnaby, BC, Canada)
T cells were isolated from the PBMCs using CD3+ Dynabeads (Life Technologies, Burlington, ON, Canada)

controls

The protocol was performed as previously described in the linked publication (link), which may have included positive and/or negative controls, but they are not explicitly mentioned in the current input.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!