All chemicals and solvents were of a reagent or HPLC grade and used without further purification. Amino acid monomers N-α-Fmoc-L-aspartic acid β-t-butyl ester (Fmoc-Asp(OtBu)-OH), N-α-Fmoc-L-glutamic acid γ-t-butyl ester (Fmoc-Glu(OtBu)-OH), and N-α-Fmoc-O-t-butyl-L-serine (Fmoc-Ser(tBu)-OH) were used. The amino acids, Wang resin, N,N ′diisopropylcarbodiimide (DIPCI), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU), 1-hydroxy benzotriazole monohydrate (HOBt), N,Ndiisopropylethylamine (DIEA), trifluoroacetic acid (TFA), and piperidine were purchased from Watanabe Chemical Industries, Ltd. (Watanabe, Hiroshima, Japan). N,N-Dimethyl-4aminopyridine (DMAP) was purchased from Tokyo Chemical Industry Co., Ltd. (TCI, Tokyo, Japan). N-Methylpyrrolidone (NMP), triisopropylsilane, thioanisole, and diethyl ether were purchased from FUJIFILM Wako Pure Chemical Corporation (Wako, Osaka, Japan).
Protocol detail
Peptide Synthesis Using Fmoc Chemistry
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Corresponding Organization : Konan University
Other organizations : Kobe Medical Center, Ryukoku University
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Variable analysis
independent variables
- Amino acid monomers N-α-Fmoc-L-aspartic acid β-t-butyl ester (Fmoc-Asp(OtBu)-OH)
- N-α-Fmoc-L-glutamic acid γ-t-butyl ester (Fmoc-Glu(OtBu)-OH)
- N-α-Fmoc-O-t-butyl-L-serine (Fmoc-Ser(tBu)-OH)
- Not explicitly mentioned
- All chemicals and solvents were of a reagent or HPLC grade and used without further purification
- Wang resin
- N,N′diisopropylcarbodiimide (DIPCI)
- 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU)
- 1-hydroxy benzotriazole monohydrate (HOBt)
- N,Ndiisopropylethylamine (DIEA)
- Trifluoroacetic acid (TFA)
- Piperidine
- N,N-Dimethyl-4aminopyridine (DMAP)
- N-Methylpyrrolidone (NMP)
- Triisopropylsilane
- Thioanisole
- Diethyl ether
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