The aseptic nodal segments (1.0 cm long) of 7 days old in vitro were placed on MS medium supplemented with 6-benzylaminopurine (BAP) at different concentrations (0.0, 1.0 and 2.0 mg/L) either singly or in combination with α-Naphthalene acetic acid (NAA; 0.0, 1.0, 2.0 and 3.0 mg/L). The maximum percentage of shoot proliferation, number of shoots per explant, shoot length (mm), and number of leaves per explant were recorded and compared statistically after 45 days of culture (starting from the initial day of inoculation). A set of cultures without plant growth regulators (PGRs) served as the control.
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In Vitro Shoot Proliferation Optimization
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Corresponding Organization :
Other organizations : Prince of Songkla University, Nakhon Si Thammarat Rajabhat University, Bansomdejchaopraya Rajabhat University, Xishuangbanna Tropical Botanical Garden
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Variable analysis
independent variables
- Concentration of 6-benzylaminopurine (BAP) (0.0, 1.0 and 2.0 mg/L)
- Concentration of α-Naphthalene acetic acid (NAA) (0.0, 1.0, 2.0 and 3.0 mg/L)
- Percentage of shoot proliferation
- Number of shoots per explant
- Shoot length (mm)
- Number of leaves per explant
- Aseptic nodal segments (1.0 cm long) of 7 days old in vitro
- MS medium as the culture medium
- Positive control: A set of cultures without plant growth regulators (PGRs)
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