Measuring TGF-β1 and PDGF-BB in Platelets

TGF-β1 and PDGF-BB were measured after activation in both PPP and PRP fractions to assess the effect of the centrifugation in PLTs and associated GFs. TGF-β1 and PDGF-BB were measured using ELISA kits (Human TGF-beta 1 DuoSet ELISA de R&D Systems DY240-05 and Human PDGF-BB DuoSet ELISA de R&D Systems DY220, respectively) according to manufacturer's instructions. These GFs were determined using human antibodies because these kits have been used for the same purposes in other feline PC studies (20 (link), 44 (link)) since it has been reported that human and cat PDGF-BB present high peptide sequence homology (45 (link)).

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Miguel-Pastor L., Satué K., Chicharro D., Torres-Torrillas M., del Romero A., Peláez P., Carrillo J.M., Cuervo B., Sopena J.J., Cerón J.J, & Rubio M. (2022). Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study. Frontiers in Veterinary Science, 9, 866547.

Publication 2022

Human TGF-beta 1 DuoSet ELISA DY240-05 Human PDGF-BB DuoSet ELISA DY220

Antibodies Centrifugation Elisa Feline Human Pdgf bb Peptide Tgf beta 1

Corresponding Organization : Universidad Cardenal Herrera CEU

Other organizations : Universidad de Murcia

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Centrifugation

dependent variables

TGF-β1 concentration
PDGF-BB concentration

control variables

Use of human antibodies for ELISA kits to measure TGF-β1 and PDGF-BB
Measuring TGF-β1 and PDGF-BB in both PPP and PRP fractions

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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