Western Blot Analysis of Hepatocyte Proteins

Total protein lysate was extracted from hepatocytes and liver tissue using lysis buffer (Cell Signaling Technology) and western blotting as previously described6 (link). P38, phospho-P38, ERK, phospho-ERK, JNK, phospho-JNK, caspase 3, cleaved caspase 3, BCL-2, IRF1, and RIG-I were purchased from Cell Signaling Technology; ADAR1 antibody was purchased from Santa Cruz (cat# SC73408, clone 15.8.6). Secondary antibodies were purchased from Promega.

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Wang H., Wang G., Zhang L., Zhang J., Zhang J., Wang Q, & Billiar T.R. (2016). ADAR1 Suppresses the Activation of Cytosolic RNA-Sensing Signaling Pathways to Protect the Liver from Ischemia/Reperfusion Injury. Scientific Reports, 6, 20248.

Publication 2016

lysis buffer phospho-P38 phospho-ERK phospho-JNK caspase 3 cleaved caspase 3 BCL-2 RIG-I ADAR1 antibody

Antibodies Antibody Bcl 2 Buffer Caspase 3 Clone Hepatocytes Irf1 Liver Promega Protein Rig i Tissue

Corresponding Organization :

Other organizations : Huazhong University of Science and Technology, UPMC Presbyterian, University of Pittsburgh Medical Center, Presbyterian Hospital, Union Hospital, Third Xiangya Hospital, Central South University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Phospho-P38
Phospho-ERK
Phospho-JNK
Caspase 3
Cleaved caspase 3
BCL-2
RIG-I
ADAR1

control variables

Hepatocytes and liver tissue
Lysis buffer (Cell Signaling Technology)
Western blotting as previously described6 (link)
Secondary antibodies purchased from Promega

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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