Protocol detail

Quantification of Zinc in Cells

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The protocols for detection of Zn content in cell were reported in our previous paper [41 (link)]. Briefly, isolated GCs were cultured in 6-well plates for 2 days then the cells were treated with different concentration of ZnO NP or ZnSO₄ for 24h. About 2×10⁶ cells per treatment were collected for determination of Zn in the cells. 500μL of 0.4% Triton X-100 in PBS was used to lyse the cells and the lysate was diluted to 2mL with 0.1% Triton X-100. Samples were analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES, Optima 2100, Perkin-Elmer, Shelton, CT, USA) [46 ].

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Zhao Y., Li L., Min L.J., Zhu L.Q., Sun Q.Y., Zhang H.F., Liu X.Q., Zhang W.D., Ge W., Wang J.J., Liu J.C, & Hao Z.H. (2016). Regulation of MicroRNAs, and the Correlations of MicroRNAs and Their Targeted Genes by Zinc Oxide Nanoparticles in Ovarian Granulosa Cells. PLoS ONE, 11(5), e0155865.

Publication 2016

Optima 2100

Cells Emission Plasma Spectroscopy Triton x 100

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Variable analysis

independent variables

Concentration of ZnO NP
Concentration of ZnSO4

dependent variables

Zinc content in cells

control variables

Time of cell treatment (24h)
Cell type (isolated GCs)
Cell culture duration (2 days)
Cell density (2x10^6 cells per treatment)
Lysis buffer (0.4% Triton X-100 in PBS, diluted to 0.1% Triton X-100)
Analytical method (ICP-OES)

controls

No positive control mentioned
No negative control mentioned

Annotations

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