Rats were anaesthetized with ketamine-xylazine at the end of the experiment, and blood samples were collected via cardiac puncture for hematology and serum biochemistry analyses. Blood serum for biochemistry analysis was collected in non-heparinized tubes. Collected blood was left at room temperature for a while to allow clotting before serum collection by centrifugation (3000× g for 10 min) at 4 °C. The collected serum was stored at −4 °C before analysis using an automatic clinical chemistry analyzer (Hitachi, Japan). The parameters analyzed for serum biochemistry analysis were as follows: albumin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine, total bilirubin, urea, and total protein.
Blood was collected in K2EDTA tubes using an automated hematology analyzer (CELL-- DYN 3700 Abbott Diagnostics, Des Plaines, IL, USA). Parameters analyzed for whole blood cells were as follows: red blood cell count, hemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concentration, thrombocytes, and white blood cell count (including lymphocytes, monocytes, neutrophils, and eosinophils) [24 (link)].
Blood was collected in K2EDTA tubes using an automated hematology analyzer (CELL-- DYN 3700 Abbott Diagnostics, Des Plaines, IL, USA). Parameters analyzed for whole blood cells were as follows: red blood cell count, hemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concentration, thrombocytes, and white blood cell count (including lymphocytes, monocytes, neutrophils, and eosinophils) [24 (link)].
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