Induction and Measurement of Motor-Evoked Potentials in Rats

MEPs were induced according to the previous method [26 (link), 27 (link)]. Briefly, rats were anesthetized with 1% pentobarbital sodium (20 mg/kg i.p.). The stimulation needle electrode (DSN1620, Medtronic, USA), acting as the anode, was inserted subcutaneously at the base of the nose with the tip point touching the scalp. The needle electrode acting as the cathode was placed subcutaneously at the midpoint of the two ears. The recorded electrode was inserted into the tibialis anterior (TA) muscle. A ground electrode was placed subcutaneously at the base of the tail. A single pulse of electrical stimulation (10 mA, 0.1 ms, 1 Hz) to excite the brain was delivered via stimulator (Keypoint, Medtronic, USA). The electrical stimulation was repeated five times at an interval of 15 s in each rat. The base-to-peak amplitude of the trace after single stimulation was recorded for each type of evoked potential.

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Chen B., Tan Q., Zhao W., Yang Q., Zhang H., Gao F., Liu X., Feng H, & Jiang D. (2020). Diffusion tensor imaging and electrophysiology as robust assays to evaluate the severity of acute spinal cord injury in rats. BMC Neurology, 20, 236.

Publication 2020

DSN1620 Keypoint

Brain Ears Electrical stimulation Evoked potential Meps Needle Nose Pentobarbital sodium Pulse Scalp Tail Tibialis anterior muscle

Corresponding Organization : Chongqing Medical University

Other organizations : First Affiliated Hospital of Chongqing Medical University, Southwest Hospital, Army Medical University, Sichuan University

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Variable analysis

independent variables

Electrical stimulation (10 mA, 0.1 ms, 1 Hz) to excite the brain

dependent variables

Base-to-peak amplitude of the trace after single stimulation

control variables

Anesthesia (1% pentobarbital sodium, 20 mg/kg i.p.)
Stimulation needle electrode (DSN1620, Medtronic, USA) as the anode, inserted subcutaneously at the base of the nose
Needle electrode as the cathode, placed subcutaneously at the midpoint of the two ears
Recorded electrode inserted into the tibialis anterior (TA) muscle
Ground electrode placed subcutaneously at the base of the tail
Electrical stimulation repeated five times at an interval of 15 s in each rat

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