Purification and Antibody Validation Protocol

Gentisyl alcohol was purified from a culture of Arthrinium spp., KUC21332, as described previously [10 (link)]. Antibodies against phosphorylated ERK1/2 (Thr²⁰²/Tyr²⁰⁴, catalog number: 9101), JNK (Thr¹⁸³/Tyr¹⁸⁵, catalog number: 4668), P38 (Thr¹⁸⁰/Tyr¹⁸², catalog number: 4511), P90RSK (Thr⁵⁷³, catalog number: 9346), AKT (Ser⁴⁷³, catalog number: 4060), P70S6K (Thr⁴²¹/Ser⁴²⁴, catalog number: 9204), S6 (Ser^235/236, catalog number: 2211), and GSK3β (Ser⁹, catalog number: 9336). Antibodies against total ERK1/2 (catalog number: 4695), JNK (catalog number: 9252), P38 (catalog number: 9212), P90RSK (catalog number: 9335), AKT (catalog number: 9272), P70S6K (catalog number: 9202), S6 (catalog number: 2217), GSK3β (catalog number: 9315) were purchased from Cell Signaling Technology (Beverly, MA, USA). The inhibitors for ERK1/2 (U0126), PI3K/AKT (LY294002), and JNK (SP600125) were obtained from Enzo Life Sciences, Inc. (Farmingdale, NY, USA).

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Ham J., Lim W., Kim K., Heo Y.M., Ryu S.M., Lee D., Kim J.J, & Song G. (2019). Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer Cells. Marine Drugs, 17(6), 331.

Publication 2019

P90RSK P70S6K GSK3β U0126 (LY294002),

Antibodies Erk1 Gentisyl alcohol Gsk3β Inhibitors Ly294002 P70s6k P90rsk Pi3k Sp600125 U0126

Corresponding Organization : Korea University

Other organizations : Kookmin University, Korea Institute of Oriental Medicine

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Variable analysis

independent variables

Gentisyl alcohol purified from a culture of Arthrinium spp., KUC21332

dependent variables

Phosphorylation of ERK1/2 (Thr^202^/Tyr^204^)
Phosphorylation of JNK (Thr^183^/Tyr^185^)
Phosphorylation of P38 (Thr^180^/Tyr^182^)
Phosphorylation of P90RSK (Thr^573^)
Phosphorylation of AKT (Ser^473^)
Phosphorylation of P70S6K (Thr^421^/Ser^424^)
Phosphorylation of S6 (Ser^235/236^)
Phosphorylation of GSK3β (Ser^9^)

control variables

Total ERK1/2
Total JNK
Total P38
Total P90RSK
Total AKT
Total P70S6K
Total S6
Total GSK3β

Annotations

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