Immunoblot analysis of liver proteins

Protein concentration of each liver sample was determined as previously described60 (link). The protocol for immunoblot analysis was also previously described60 (link). The following antibodies were used in this study: Complement C5 (Cat. No. bs-15197R, Beijing Biosynthesis Biotechnology Co., Ltd. Beijing, China), TNFα (Cat. No. bs-10802R, Beijing Biosynthesis Biotechnology Co., Ltd. Beijing, China) and β-Actin (Cat. No. sc-47778; Santa Cruz Biotechnology, Inc., Santa Cruz, CA).

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Liu L., Zhao X., Wang Q., Sun X., Xia L., Wang Q., Yang B., Zhang Y., Montgomery S., Meng H., Geng T, & Gong D. (2016). Prosteatotic and Protective Components in a Unique Model of Fatty Liver: Gut Microbiota and Suppressed Complement System. Scientific Reports, 6, 31763.

Publication 2016

TNFα β-Actin

Actin Antibodies Biosynthesis Complement c5 Immunoblot analysis Liver Protein Tnfα

Corresponding Organization :

Other organizations : Yangzhou University, University of British Columbia, Shanghai Jiao Tong University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein concentration of each liver sample
Protein expression levels of Complement C5, TNFα, and β-Actin

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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