Subcellular Localization of MeSTP Genes

The ORFs of MeSTP genes were amplified using gene-specific primers (Supplementary Table S3) and then cloned into pX-DG vector fused with GFP (green fluorescent protein) according to Chen et al. [55 (link)]. The plasmid constructs were then transiently expressed in cassava mesophyll protoplasts as previously described by Wu et al. [56 (link)]. The GFP fluorescence was imaged using a confocal laser scanning microscope (Leica TCS SPII, Leica Microsystems, Wetzlar, Germany).

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Liu Q., Dang H., Chen Z., Wu J., Chen Y., Chen S, & Luo L. (2018). Genome-Wide Identification, Expression, and Functional Analysis of the Sugar Transporter Gene Family in Cassava (Manihot esculenta). International Journal of Molecular Sciences, 19(4), 987.

Publication 2018

Leica TCS SPII

Cassava Confocal laser scanning microscope Fluorescence Gene Orfs Plasmid Primers Protoplasts Vector

Corresponding Organization : Hainan University

Other organizations : Tropical Crops Genetic Resources Institute

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Variable analysis

independent variables

ORFs of MeSTP genes

dependent variables

GFP fluorescence

control variables

Cassava mesophyll protoplasts
Confocal laser scanning microscope (Leica TCS SPII, Leica Microsystems, Wetzlar, Germany)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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