Lipoprotein Fractionation and Quantification

Lipoproteins were separated from 2.5 μL of individual plasma samples by size-exclusion chromatography using a Superose 6 PC 3.2/30 column (GE Healthcare BioSciences AB). The sephadex column separated the lipoproteins into fractions of very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL). Triglycerides (TG) and total cholesterol concentrations were calculated after integration of the individual chromatograms [21 (link), 22 (link)], generated by the enzymatic-colorimetric reaction (Cholesterol CHOD-PAP and TG GPO-PAP kits, Roche Diagnostics).

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Bandaru S., Ala C., Ekstrand M., Akula M.K., Pedrelli M., Liu X., Bergström G., Håversen L., Borén J., Bergo M.O, & Akyürek L.M. (2020). Lack of RAC1 in macrophages protects against atherosclerosis. PLoS ONE, 15(9), e0239284.

Publication 2020

Superose 6 PC 3.2/30 column

Chod Cholesterol Colorimetric Diagnostics Enzymatic High density lipoproteins Lipoproteins Low density lipoproteins Plasma Sephadex Size exclusion chromatography Triglycerides Very low density lipoproteins

Corresponding Organization : Sahlgrenska University Hospital

Other organizations : Karolinska Institutet

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Variable analysis

independent variables

Size-exclusion chromatography using a Superose 6 PC 3.2/30 column (GE Healthcare BioSciences AB)

dependent variables

Triglycerides (TG) and total cholesterol concentrations

control variables

2.5 μL of individual plasma samples

controls

Positive control: Enzymatic-colorimetric reaction (Cholesterol CHOD-PAP and TG GPO-PAP kits, Roche Diagnostics)

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