The protein extraction method and detailed steps of western blotting were adapted from a previous study [23 (link)]. The following antibodies were used: TCF21 and cleaved caspase 3 from Abcam (Britain) and p-AKT, AKT, caspase 3, BCL-xL, TFAP2A, and GAPDH from Proteintech (Wuhan, China).
Free full text: Click here