Prior to conditioning, subjects were given food without Doxycycline for 2 days. During fear conditioning, subjects were placed in a shock chamber (30 × 35 × 32 cm) scented with 0.25% benzaldehyde in 70% ethanol for 500 s. (Ctx A). Foot shocks (0.75 mA, 2 s duration) were administered at the198 s, 278 s, 358 s, and 438 s time points. For reward conditioning, each subject was exposed to a single female mouse for 2 h in their home cage in a room separate from the housing room (Ctx B). Immediately after, in both fear and reward conditioning, subjects returned to a Dox (40 mg/kg) diet.
Protocol detail
Pavlovian Conditioning in Mice
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Corresponding Organization :
Other organizations : Massachusetts Institute of Technology, Howard Hughes Medical Institute
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Variable analysis
independent variables
- Presence or absence of Doxycycline in the diet (40 mg/kg)
- Freezing behavior during fear conditioning in the shock chamber (Ctx A)
- Preference for female mouse during reward conditioning (Ctx B)
- Size of shock chamber (30 × 35 × 32 cm)
- Concentration of benzaldehyde in 70% ethanol (0.25%)
- Intensity of foot shocks (0.75 mA, 2 s duration)
- Duration of fear conditioning (500 s)
- Time points of foot shocks (198 s, 278 s, 358 s, 438 s)
- Duration of reward conditioning (2 h)
- Positive control: Subjects were given food without Doxycycline for 2 days prior to conditioning, which serves as a baseline condition to compare the effects of Doxycycline.
- Negative control: Not explicitly mentioned in the provided information.
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