Cytotoxicity Evaluation of Mushroom Extracts

Cytotoxicity against human leukemia (CCRF-CEM), acute promyelocytic leukemia (NB4), acute monocytic leukemia (THP1), and human lymphoma (U937) was determined using the Abcam^® Water Soluble Tetrazolium Salts (WST-1) assay kit (ab155902 WST-1 Cell Proliferation Reagent, UK). Cell suspensions were incubated for 24 h, and then treated with different concentrations (0 to 100 μg/mL in complete media) of mushroom extracts for 48 h. Cells were then treated with 10 μL WST-1 reagent for 1 h, then A₄₅₀ was read using a microplate reader [38 (link)].

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Elhusseiny S.M., El-Mahdy T.S., Awad M.F., Elleboudy N.S., Farag M.M., Aboshanab K.M, & Yassien M.A. (2021). Antiviral, Cytotoxic, and Antioxidant Activities of Three Edible Agaricomycetes Mushrooms: Pleurotus columbinus, Pleurotus sajor-caju, and Agaricus bisporus. Journal of Fungi, 7(8), 645.

Publication 2021

WST-1) assay kit

Acute monocytic leukemia Acute promyelocytic leukemia Assay Cell proliferation Cells Cytotoxicity Human Leukemia Lymphoma Mushroom Tetrazolium salts

Corresponding Organization : Ain Shams University

Other organizations : Ahram Canadian University, Helwan University, Taif University, Al-Azhar University

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Variable analysis

independent variables

Concentration of mushroom extracts (0 to 100 μg/mL in complete media)

dependent variables

Cytotoxicity against human leukemia (CCRF-CEM)
Cytotoxicity against acute promyelocytic leukemia (NB4)
Cytotoxicity against acute monocytic leukemia (THP1)
Cytotoxicity against human lymphoma (U937)

control variables

Cell suspensions incubated for 24 h
Cells treated with mushroom extracts for 48 h
Cells treated with 10 μL WST-1 reagent for 1 h
A450 read using a microplate reader

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