Photosynthesis and Leaf Stress Responses

Photosynthesis of the leaf and the water content was tested. Net photosynthetic rate, intercellular carbon dioxide concentration and stomatal conductance were measured by a portable photosynthesis system (LI-6800; LI-COR, Lincoln, NE, USA). For leaf chamber environment, chamber temperature, relative humidity and CO₂ concentration were set at 25 °C, 70%, and 400 μmol mol⁻¹·s⁻¹, each experiment was repeated 10 times. MDA content was analyzed through the TAB method (Banga & Lengyel, 1980) with MDA assay kit (sigma). Proline content, antioxidant capacity and ion content were measured as previously described [62 (link)] with test kits (Jiancheng Bioengineering Institute, Nanjing, China). Element content analysis was performed using inductively coupled plasma-optical emission spectrophotometry ICP-OES (ICAP 7000, Thermo Scientific, Waltham, MA, USA).