Comprehensive Multiparameter Analysis of Antigen-Specific T Cells
Corresponding Organization : National Research Council
Other organizations : Sapienza University of Rome, Italian Institute of Technology, The Francis Crick Institute, University of Naples Federico II, Ceinge Biotecnologie Avanzate (Italy), Istituto Neurologico Mediterraneo, King's College London, Guy's and St Thomas' NHS Foundation Trust, National Institute for Health Research
Variable analysis
- Incubation of cells with purified anti-mouse CD16/CD32 clone 2.4G2 (Fc block)
- Expression of H-2k(d) AMQMLKETI (gag197-205) MHC class I tetramer on cells
- Expression of gag197-205 pentamer on cells
- Expression of CD3, CD8, CD127, CD62L surface molecules on cells
- Expression of Ki-67 intracellular molecule on cells
- Hoechst 33342 dye for excluding dead cells
- No positive or negative controls were explicitly mentioned in the input protocol.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!