Cells were incubated with purified anti-mouse CD16/CD32 clone 2.4G2 (Fc block; BD Biosciences, San Jose, CA, USA), and stained as described with H-2k(d) AMQMLKETI (gag197-205) allophycocyanin (APC)-labeled Tetramer (Tetr-gag, NIH Tetramer Core Facility, Atlanta, GA, USA) and phycoerythrin (PE)-labeled Pentamer (Pent-gag, Proimmune, Oxford, UK), fluorochrome conjugated monoclonal Antibodies (mAbs) against surface (CD3, CD8, CD127, CD62L) and intracellular (Ki-67) molecules, and Hoechst 33342 (Thermo Fisher Scientific, Waltham, MA, USA) (14 (link)). The following mAbs were used: anti-CD3ε peridinin chlorophyll protein (PerCP)-Cy5.5 (clone 145-2C11, BD Biosciences), anti-CD8α BUV805 (clone 53-6.7, BD Biosciences), anti-CD127 biotin (clone A7R34, eBioscience, Thermo Fisher Scientific) plus Streptavidin PE-Cy7 (BD Biosciences), or anti-CD62L PE-Cy7 (clone MEL-14, Biolegend, San Diego, CA, USA), and anti-Ki-67 mAb conjugated with Fluorescein isothiocyanate (FITC) or Alexafluor 700 (clone SolA-15; eBioscience, Thermo Fisher Scientific). Dead cells were excluded with eBioscience Fixable Viability Dye eFluor780 (eFluor780, Invitrogen, Thermo Fisher Scientific)
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