Cell growth was detected by measuring OD600 using a spectrophotometer (UVmini-1240, Shimadzu Corporation, Japan).
Naringenin, eriodictyol, dihydrokaempferol, dihydroquercetin, kaempferol, quercetin, apigenin, luteolin, daidzein, and 7,3′,4′-trihydroxyisoflavone were quantified using an Agilent 1260 HPLC instrument (Agilent Technologies, Santa Clara, CA, United States) equipped with ultraviolet/VIS detector. A reverse-phase column ZORBAX Eclipse XDB-C18 (5 μm, 4.6 mm × 250 mm, Agilent, United States) was used to monitor the absorbance at 290 nm. Elution was performed with mobile phase A consisting water containing 0.1% trifluoroacetic acid and mobile phase B consisting methanol containing 0.1% trifluoroacetic acid. The flow rate was set as 0.8 ml·min-1 and the solvent gradient was adopted as follow: 0–1 min, isocratic at 10% B; 1–10 min, 10%–40% B; 10–20 min, 40%–60% B; 20–23 min, 60% B; 23–25 min, 60%–10% B; 25–27 min, 10% B.
Naringenin, eriodictyol, dihydrokaempferol, dihydroquercetin, kaempferol, quercetin, apigenin, luteolin, daidzein, and 7,3′,4′-trihydroxyisoflavone were quantified using an Agilent 1260 HPLC instrument (Agilent Technologies, Santa Clara, CA, United States) equipped with ultraviolet/VIS detector. A reverse-phase column ZORBAX Eclipse XDB-C18 (5 μm, 4.6 mm × 250 mm, Agilent, United States) was used to monitor the absorbance at 290 nm. Elution was performed with mobile phase A consisting water containing 0.1% trifluoroacetic acid and mobile phase B consisting methanol containing 0.1% trifluoroacetic acid. The flow rate was set as 0.8 ml·min-1 and the solvent gradient was adopted as follow: 0–1 min, isocratic at 10% B; 1–10 min, 10%–40% B; 10–20 min, 40%–60% B; 20–23 min, 60% B; 23–25 min, 60%–10% B; 25–27 min, 10% B.
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