Samples were prepared using a RIPA lysis buffer containing a protease inhibitor cocktail. The protein concentrations were quantified with the BCA kit (All kits from KeyGen Biotech). Subsequent experiment was conducted as recently described (26 (link)). The antibodies used for western blotting were as follows: Rabbit anti-BCL11A (ab191401, 1:10,000, Abcam), rabbit anti-AR (ab74272, 1:300, Abcam), rabbit anti-GAPDH (ab181603, 1:10,000, Abcam) and rabbit IgG (#7074, 1:10,000, Cell Signaling Technologies, Inc.).
Protocol detail
Protein Quantification and Western Blotting
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Corresponding Organization :
Other organizations : Nanjing General Hospital of Nanjing Military Command, Nanjing University, Nanjing Medical University, Zhongshan People's Hospital, Jiangsu Province Hospital, Jiangsu University, Affiliated Hospital of Jiangsu University
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Variable analysis
independent variables
- RIPA lysis buffer
- Protease inhibitor cocktail
- Protein concentrations
- BCA kit
- Rabbit anti-BCL11A (ab191401, 1:10,000, Abcam)
- Rabbit anti-AR (ab74272, 1:300, Abcam)
- Rabbit anti-GAPDH (ab181603, 1:10,000, Abcam)
- Rabbit IgG (#7074, 1:10,000, Cell Signaling Technologies, Inc.)
- Rabbit IgG (#7074, 1:10,000, Cell Signaling Technologies, Inc.)
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