Three clinical strains of Af, isolated from respiratory samples, were used in the present study. Identification was confirmed by sequencing part of the gene encoding beta-tubulin. The CYP51A gene and its promoter had been previously sequenced to determine the mutations involved in azole-resistance (Jemel et al., 2021 (link)). We included one azole-susceptible strain (AfS) with a wild type CYP51A sequence, one strain (AfR1) with a G54W mutation and one strain (AfR2) with a L98H point mutation in CYP51A in combination with a 34-bp tandem repeat in the promoter (TR34/L98H).
Subcultures were performed on Sabouraud dextrose agar (VWR, Fontenay-sous-bois, France) with chloramphenicol (Sigma-Aldrich, Saint Quentin-Fallavier, France). They were incubated for 7 days at 37°C to obtain sufficient sporulation.
Subcultures were performed on Sabouraud dextrose agar (VWR, Fontenay-sous-bois, France) with chloramphenicol (Sigma-Aldrich, Saint Quentin-Fallavier, France). They were incubated for 7 days at 37°C to obtain sufficient sporulation.
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