Fasting Protocol for Behavioral Studies in Mice

As we have described (22 (link)), 2 days prior to fasting, mice were singly housed. Fasting was initiated by placing mice in a new cage without food but with ad libitum water. Mice were fasted for 24 h starting at 09:00 h. Immediately after the 24 h fasting, behavioral testing, biomarker studies, and/or injection studies were performed. Consequently, all studies occurred during the dark (active) cycle. Mouse weight was recorded immediately before and immediately after fasting using an Ohaus Adventurer Pro digital scale (Parsippany, NJ, USA).

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Joesting J.J., Moon M.L., Gainey S.J., Tisza B.L., Blevins N.A, & Freund G.G. (2014). Fasting Induces IL-1 Resistance and Free-Fatty Acid-Mediated Up-Regulation of IL-1R2 and IL-1RA. Frontiers in Immunology, 5, 315.

Publication 2014

Adventurer Pro digital scale

Biomarker Food Mouse

Corresponding Organization : University of Illinois Urbana-Champaign

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Variable analysis

independent variables

Fasting duration (24 h)

dependent variables

Mouse weight (immediately before and after fasting)

control variables

Housing condition (singly housed)
Water availability (ad libitum)
Timing of fasting and behavioral testing (during dark/active cycle)

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