Cytospin and Immunostaining Protocol

Cytospin of FACS-purified cells was prepared as described (60 (link)) using Shandon Cytospin 4 (Thermo Scientific). Giemsa-Wright staining was performed using Advia S60 (Siemens) and imaged using Axioimager.Z2 microscope with Axiovision software v4.8 (Carl Zeiss, Germany). Human tonsil paraffin sections were immunostained with the antibodies: anti-AXL (MM0098-2N33, Abcam), CD123 (BR4MS, Leica Biosystems) and CD3 (LN10, Leica Biosystems) using a Ventana Benchmark XT instrument.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Villani A.C., Satija R., Reynolds G., Sarkizova S., Shekhar K., Fletcher J., Griesbeck M., Butler A., Zheng S., Lazo S., Jardine L., Dixon D., Stephenson E., Nilsson E., Grundberg I., McDonald D., Filby A., Li W., De Jager P.L., Rozenblatt-Rosen O., Lane A.A., Haniffa M., Regev A, & Hacohen N. (2017). Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes and progenitors. Science (New York, N.Y.), 356(6335), eaah4573.

Publication 2017

Shandon Cytospin 4 Axioimager.Z2 microscope Axiovision software v4.8 CD3 (LN10,

Antibodies Cd123 Cells Giemsa Human Microscope Paraffin Tonsil

Corresponding Organization : Howard Hughes Medical Institute

Other organizations : Ragon Institute of MGH, MIT and Harvard, Dana-Farber Cancer Institute, Harvard University, Olink (United States), Brigham and Women's Hospital

Top 5 similar protocols

Variable analysis

independent variables

Cytospin of FACS-purified cells

dependent variables

Giemsa-Wright staining
Imaging using Axioimager.Z2 microscope with Axiovision software v4.8

control variables

Shandon Cytospin 4 (Thermo Scientific)
Advia S60 (Siemens)
Ventana Benchmark XT instrument

controls

Human tonsil paraffin sections were used as a control for immunostaining with the antibodies: anti-AXL (MM0098-2N33, Abcam), CD123 (BR4MS, Leica Biosystems) and CD3 (LN10, Leica Biosystems)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!