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Bacterial and Yeast Strain Preparation

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Strains and plasmids used in this work are shown in Table 1. E. coli strains were grown in LB medium containing 30 μg/mL kanamycin. S.cerevisiae strains were prepared as previously described [19 (link)], and were grown in synthetic minimal (SD) medium containing 0.67% yeast nitrogen base (Becton-Dickinson), amino acid supplements (Sunrise), and 2% glucose. Expression in E. coli was induced by adding IPTG at OD₆₀₀ of 0.4.

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Zhu J., Lin J.L., Palomec L, & Wheeldon I. (2015). Microbial host selection affects intracellular localization and activity of alcohol-O-acetyltransferase. Microbial Cell Factories, 14, 35.

Publication 2015

yeast nitrogen base

Amino acid E coli Glucose Iptg Kanamycin Nitrogen Plasmids Strains Supplements Yeast

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Variable analysis

independent variables

IPTG induction in E. coli

dependent variables

Expression of proteins in E. coli
Growth of S. cerevisiae strains

control variables

LB medium containing 30 μg/mL kanamycin for E. coli strains
Synthetic minimal (SD) medium containing 0.67% yeast nitrogen base, amino acid supplements, and 2% glucose for S. cerevisiae strains

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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