Quantifying Inflammatory Cell Infiltration via MPO ELISA

Inflammatory cell infiltration was estimated by quantifying myeloperoxidase (MPO) using a sandwich ELISA (Hycult Biotech, Plymouth Meeting PA), as previously described. At 10 h postinfection, eyes were harvested, transferred into PBS supplemented with proteinase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN) and homogenized using 1-mm sterile glass beads (BioSpec Products, Inc.). Uninfected eye homogenates were the negative controls. The lower limit of detection for this assay was 2 ng/ml (73 (link), 89 (link), 91 (link), 96 (link)–98 (link)).

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Mursalin M.H., Coburn P.S., Livingston E., Miller F.C., Astley R., Flores-Mireles A.L, & Callegan M.C. (2020). Bacillus S-Layer-Mediated Innate Interactions During Endophthalmitis. Frontiers in Immunology, 11, 215.

Publication 2020

sandwich ELISA proteinase inhibitor cocktail 1-mm sterile glass beads

Assay Cell Diagnostics Elisa Inflammatory Myeloperoxidase Proteinase inhibitor Sterile

Corresponding Organization : Dean McGee Eye Institute

Other organizations : University of Notre Dame

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Inflammatory cell infiltration, as measured by quantifying myeloperoxidase (MPO) using a sandwich ELISA

control variables

Uninfected eye homogenates used as negative controls

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