RNA Isolation and Plasmid Generation

RaPure Total RNA kit (Magen) was used to isolate total RNA according to the manufacturer's instructions. cDNA was synthesized using reverse transcriptase (Promega). CUL4A, CUL4B, DDB1, MMS22L and ESCO2 plasmids for transient expression were generated previously (22 (link)). GSK3A and GSK3B full-length genes were generated by PCR and were inserted into the pRK5-FLAG vector. All genes were sub-cloned into other vectors when necessary. See Supplementary Table S2 for details of plasmids used in this study, Supplementary Table S3 for plasmids generated in this study, and Supplementary Table S4 for primers used in this study.

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Zhang J., Li L., Miao Y., Liu X., Sun H., Jiang M., Li X., Li Z., Liu C., Liu B., Xu X., Cao Q., Hou W., Chen C, & Lou H. (2023). Symmetric control of sister chromatid cohesion establishment. Nucleic Acids Research, 51(10), 4760-4773.

Publication 2023

RaPure Total RNA kit reverse transcriptase

Cdna Ddb1 Genes Plasmids Primers Promega Reverse transcriptase Transient Vectors

Corresponding Organization : China Agricultural University

Other organizations : Tsinghua University, Sichuan University, University of Hong Kong

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Variable analysis

independent variables

CUL4A, CUL4B, DDB1, MMS22L and ESCO2 plasmids for transient expression
GSK3A and GSK3B full-length genes

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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