Fungal DNA Extraction from Compost

Fungal DNA from compost was extracted using a modified version of Sturrock et al. (2015) (link). Compost from two biological replicate plants were combined into one sample (2 g) for each treatment of each variety. For homogenization, each sample was placed in a 50 ml falcon tube with three 1/4 inch ceramic balls (MP Biomedicals, United States), 15 ml of CTAB buffer (cetyltrimethylammonium bromide) and 0.45 ml of Antifoam B and homogenized using a FastPrep-24^TM homogeniser (MP Biomedicals, United States). DNA extraction was performed using the Wizard Magnetic DNA Purification System for Food as per manufacturer’s instructions (Promega Wizard Food Kit, Southampton, United Kingdom).

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Drizou F., Bruce T.J., Ray R.V, & Graham N.S. (2018). Infestation by Myzus persicae Increases Susceptibility of Brassica napus cv. “Canard” to Rhizoctonia solani AG 2-1. Frontiers in Plant Science, 9, 1903.

Publication 2018

FastPrep-24TM homogeniser Wizard Magnetic DNA Purification System for Food

Biological Buffer Cetyltrimethylammonium bromide Food Food system Fungal dna Plants Promega Replicate

Corresponding Organization : University of Nottingham

Other organizations : Keele University

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Variable analysis

independent variables

Treatment of each variety

dependent variables

Fungal DNA from compost

control variables

Compost from two biological replicate plants were combined into one sample (2 g) for each treatment of each variety
Homogenization of each sample using a 50 ml falcon tube with three 1/4 inch ceramic balls, 15 ml of CTAB buffer, and 0.45 ml of Antifoam B in a FastPrep-24TM homogeniser
DNA extraction performed using the Wizard Magnetic DNA Purification System for Food as per manufacturer's instructions (Promega Wizard Food Kit)

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