K562 and Kasumi-1 cell lines were purchased from the European Collection of Authenticated Cell Cultures (ECACC) and cultured using RPMI-1640 culture medium with L-glutamine (Sigma Aldrich, United Kingdom), further supplemented with 10% foetal bovine serum (FBS; Sigma Aldrich) for K562 cells and 20% FBS for Kasumi-1 cells. Cells were used between passages 6–19, seeded at densities of 5 × 105–1 × 106 in T25 flasks and were sub-cultured every 48 h. Cell lines were incubated in 5% CO2 at 37°C.
K562 and Kasumi-1 cells were treated with SRPK1 specific small molecule inhibitors 5-methyl-N-(2-(morpholin-4-yl)-5-(tri-fluoromethyl)phenyl) furan-2-carboxamide, commonly known as SR Protein Inhibitor X (SPHINX) which was purchased from Enamine (Kiev, Ukraine). SPHINX was dissolved in DMSO (Sigma Aldrich) at a stock concentration of 25 mM. Cells (1 × 106/ml) were seeded in a T25 flask for each treatment which was performed in duplicate. Cells were incubated with 10 nM-10 μM SPHINX for up to 72 h.
K562 and Kasumi-1 cells were treated with SRPK1 specific small molecule inhibitors 5-methyl-N-(2-(morpholin-4-yl)-5-(tri-fluoromethyl)phenyl) furan-2-carboxamide, commonly known as SR Protein Inhibitor X (SPHINX) which was purchased from Enamine (Kiev, Ukraine). SPHINX was dissolved in DMSO (Sigma Aldrich) at a stock concentration of 25 mM. Cells (1 × 106/ml) were seeded in a T25 flask for each treatment which was performed in duplicate. Cells were incubated with 10 nM-10 μM SPHINX for up to 72 h.
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