Tilapia Skin Collagen Sponge for Tissue Regeneration

Fish collagen sponge (which was extracted by acid-enzyme combination from tilapia skin (Pal et al., 2016 (link))), Bovine collagen sponge (Wuxi Beidi Bioengineering Co., LTD.), SD rat (Jinan Peng Yue Animal Breeding Center), Immunohistochemical kit (Beijing Solaibao Technology Co., LTD.), FGF antibody, VEGF antibody, Col I antibody (Abcam), Col III antibody (Amyjet), FITC (Thermo), CCK-8 kit (Beijing Solaibo Technology Co., LTD.), Live cell/dead cell double staining kit (Calcein-AM/PI), L929 (mouse fibroblast, Qingqi (Shanghai) Biotechnology Development Co., LTD.), Roswell Park Memorial Institute (RPMI) 1640 (Thermo), Glutaraldehyde (Maclin), Hematoxylin-Eosin (HE) Stain Kit (Beijing Solaibao Technology Co., LTD.), Masson’s Trichrome Stain Kit (Beijing Solaibao Technology Co., LTD.).

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Wang L., Qu Y., Li W., Wang K, & Qin S. (2023). Effects and metabolism of fish collagen sponge in repairing acute wounds of rat skin. Frontiers in Bioengineering and Biotechnology, 11, 1087139.

Publication 2023

Col I antibody FITC

Acid Animal Antibody Bovine Calcein Cck 8 Cell Collagen Enzyme Eosin Fibroblast Fish Fitc Glutaraldehyde Hematoxylin stain Mouse Skin Sponge Tilapia Trichrome stain Vegf

Corresponding Organization : Chinese Academy of Sciences

Other organizations : University of Chinese Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

Fish collagen sponge (which was extracted by acid-enzyme combination from tilapia skin (Pal et al., 2016 (link)))
Bovine collagen sponge (Wuxi Beidi Bioengineering Co., LTD.)

dependent variables

FGF antibody
VEGF antibody
Col I antibody (Abcam)
Col III antibody (Amyjet)
Cell viability (assessed by CCK-8 kit and Live cell/dead cell double staining kit (Calcein-AM/PI))
Tissue histology (assessed by Hematoxylin-Eosin (HE) Stain Kit and Masson's Trichrome Stain Kit)

control variables

SD rat (Jinan Peng Yue Animal Breeding Center)
L929 (mouse fibroblast, Qingqi (Shanghai) Biotechnology Development Co., LTD.)
Roswell Park Memorial Institute (RPMI) 1640 (Thermo)
Glutaraldehyde (Maclin)

positive controls

None specified

negative controls

None specified

Annotations

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