Isolation and Characterization of hAMSCs and hADSCs

The Ethics Committee of Hyogo College of Medicine approved this study (approval numbers: 325 and 1880). The procedures of hAMSCs and hADSCs have been described previously [11 (link), 14 (link)]. Briefly, we first obtained written informed consent from the donors; for hAMSC, pregnant women waiting for cesarean section, and for hADSCs, patients who underwent abdominal surgeries. Regarding hAMSCs, human fetal membranes were obtained by cesarean section. Amnia were detached mechanically from the chorion and digested with collagenase/dispase solution for 1 h at 37 °C in a water bath shaker. The cells were filtered through a 100-μm mesh filter, resuspended in α-minimal essential medium (α-MEM; Invitrogen, CA) supplemented with 10% bovine-derived platelet lysate “NeoSERA” (Japan Biomedical Co., Ltd, Japan), plated on dishes, and incubated at 37 °C with 5% CO₂. Spindle-shaped cells formed visible colonies in 1–2 days. Regarding hADSCs, adipose tissues were obtained during abdominal surgeries, rinsed, cut, homogenized, digested with liberase. Stromal vascular fraction was obtained, the cells were stocked at − 80 °C with STEM-CELLBANKER (Takara Bio) after the fourth passage.