Prenatal T and DHT-treated animals were generated by intramuscular administration of 100mg T propionate (1.2mg/kg; Sigma-Aldrich St. Louis, MO) and 100mg of DHT propionate (Steraloids) in 2mL of cottonseed oil (Sigma-Aldrich), respectively, to pregnant ewes twice weekly from days 30 to 90 of gestation. Ovaries were collected at four different ages, fetal days 90 (FD90) and 140 (FD140), and at postnatal 10 months (end of first breeding season; Year 1), and 21 months-of-age (end of second breeding season; Year 2). Only one female offspring from each mother were included in the study. As such the number represented also reflects number of mothers used in this study. The number of animals for FD140, Year 1 and Year 2 time points were 4 per treatment group, while at FD90, the number of animals in control and prenatal T groups were 4 and prenatal DHT group was 5. There were insufficient DHT-treated females born to include a Year 2 prenatal DHT-treated group. Developmental changes in ovarian follicular distribution determined by ovarian morphometry and changes in expression of apoptotic factors, steroid receptors, steroidogenic enzymes and ovarian VEGF and AMH patterns from the cohort used in this study have been previously published 14 (link),20 (link),38 (link)–42 (link)