Breast Cancer Cell Line Culture Protocols

MCF-7 cells were obtained from ATCC (Manassas, VA) and authenticated by STR DNA Fingerprinting at MD Anderson. Cells were cultured in DMEM (Corning, Tewksbury, MA) containing 10% FBS (R&D Systems, Inc., Minneapolis, MN) and 1% penicillin/streptomycin (Gibco, Waltham, MA) in a humidified 5% CO₂ atmosphere at 37 °C. MDA-MB-231 cells were obtained from ATCC (Manassas, VA), and authenticated by STR DNA Fingerprinting at Arizona Research labs. They were cultured in Leibovitz’s L-15 medium (Corning, Tewksbury, MA) containing 10% FBS (R&D Systems, Inc., Minneapolis, MN) in a humidified incubator at 37 °C. HCC1937, MDA-MB-468, and T-47D cell lines were obtained from ATCC (Manassas, VA) and HCC1937/WT BRCA1 (hereafter referred to as HCC1937 +) were obtained from Dr. Jensen [38 (link)]. Cell culture conditions have been previously described [38 (link)]. Cell lines were tested for mycoplasma using the ATCC Universal Mycoplasma Detection kit (Manassas, VA).