The assessment of serum calcium was routinely conducted for all of the enrolled patients within 24 h after admission. Levels of serum calcium were measured by Arsenazo III colorimetry (Beckman Coulter, Brea, America) using fresh samples, which had a functional sensitivity of 0.01 mmol/L. It was unadjusted for serum albumin and the reference interval of serum calcium for our laboratory ranged from 2.11 to 2.52 mmol/L. Hypocalcemia was defined as a serum calcium level ≤ 2.12 mmol/L [17 (link)].
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