Robust DNA Extraction from Whole Blood

DNA from WBC was either obtained from a separate EDTA test tube, when available, or from the buffy coat cells in the Streck^® tubes. DNA was isolated from 2–3.5 mL blood using EZ1 DNA Tissue Kit (Qiagen, Hilden, Germany) or on Qiasymphony (Qiagen, Hilden, Germany) at the Department of Clinical Genetics, Karolinska University Hospital, Stockholm, Sweden. A buffer change to nuclease-free water using AMPure XP according to the manufacturers’ instructions (Beckman Coulter, IN, USA) was performed before library preparation. 59–250 ng blood DNA was sequenced in the same pipeline as the cell-free DNA.