Culturing pediatric glioblastoma cell lines

A 13-year-old boy’s high-grade glioblastoma PBT24 cell line cells were donated by Prof. M. M. Alonso (University of Navarra, Spain) [43 (link)] for the study. A 3-year-old girl’s diffuse intrinsic pontine glioblastoma (DIPG) SF8628 cell line cells—harboring the histone H3.3 Lys 27-to-methionine (Sigma Aldrich, St. Louis, MO, USA)—were also studied [44 ,45 (link)]. The PBT24 cells were cultivated in Roswell Park Memorial Institute 1640 (RPMI), medium (Sigma Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS; Sigma Aldrich, St. Louis, MO, USA) containing 100 IU/mL of penicillin and 100 µg/mL of streptomycin (P/S; Sigma Aldrich, St. Louis, MO, USA). The SF8628 cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM)–High Glucose Media (Sigma Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (FBS; Sigma Aldrich, St. Louis, MO, USA) containing 100 IU/mL of penicillin and 100 µg/mL of streptomycin (P/S; Sigma Aldrich, St. Louis, MO, USA) and 2 mM L-Glutamine (Sigma Aldrich, St. Louis, MO, USA). Cells were incubated at 37 °C in a humidified 5% CO₂ atmosphere.