These were performed as described in our previous study [12] (link), [15] (link). Anti-Atp6v1c1 (H-300) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-tubulin (E7) was from the Developmental Studies Hybridoma Bank (DSHB, Iowa City, IA), and anti-β-actin monoclonal antibody (8H10D10) was purchased from Cell Signaling Technology (Danvers, MA). All assays were repeated 3 times.
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