Serum Biomarker Profiling Protocol

Serum concentrations of total cholesterol, high density lipoproteins (HDL)-cholesterol, triglycerides, immunoglobulin class G, class A and class M (IgG, IgA, IgM), were performed by a fully automatic analyzer (Cobas 6000 System; Roche Diagnostics). Total cholesterol, HDL-cholesterol, and triglycerides were assayed by using an enzymatic methods [29 (link)–31 ]. Serum fibrinogen concentration was determined by using Thromborel Reagent on BCS XP coagulation analyzer (Siemens Healthcare) [32 ]. Erythrocyte Sedimentation Rate (ESR) was assayed by using an automated system [33 (link)]. Blood cells counts were assayed on Sysmex XT-2100 system [34 (link)].

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Signorini C., De Felice C., Leoncini S., Møller R.S., Zollo G., Buoni S., Cortelazzo A., Guerranti R., Durand T., Ciccoli L., D’Esposito M., Ravn K, & Hayek J. (2016). MECP2 Duplication Syndrome: Evidence of Enhanced Oxidative Stress. A Comparison with Rett Syndrome. PLoS ONE, 11(3), e0150101.

Publication 2016

Cobas 6000 System BCS XP XT-2100 system

Blood cells Cholesterol Coagulation Diagnostics Enzymatic Erythrocyte sedimentation rate Fibrinogen High density lipoproteins cholesterol Immunoglobulin g Serum Triglycerides

Corresponding Organization : Azienda Ospedaliera Universitaria Senese

Other organizations : University of Southern Denmark, École Nationale Supérieure de Chimie de Montpellier, Centre National de la Recherche Scientifique, Institut des Biomolécules Max Mousseron, Istituto Neurologico Mediterraneo, Institute of Genetics and Biophysics, Rigshospitalet, Copenhagen University Hospital

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Serum concentrations of total cholesterol
Serum concentrations of high density lipoproteins (HDL)-cholesterol
Serum concentrations of triglycerides
Serum concentrations of immunoglobulin class G (IgG)
Serum concentrations of immunoglobulin class A (IgA)
Serum concentrations of immunoglobulin class M (IgM)
Serum fibrinogen concentration
Erythrocyte Sedimentation Rate (ESR)
Blood cells counts

control variables

Not explicitly mentioned

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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