H2O2 concentrations in PAW were quantified using the titanium oxysulfate (TiOSO4, Sigma-Aldrich, Arklow, Ireland) colorimetric method. A total of 100 µL of each sample of PAW was incubated with 10 µL TiOSO4 in the dark for ten minutes. Absorbance was read on a spectrophotometric plate reader (ThermoScientific, Waltham, MA, USA) at 405 nm wavelength. A standard curve of known H2O2 concentrations was included on each plate and used to convert absorbance into H2O2 concentration [18 (link)].
Total oxidative species in PAW were measured using the potassium iodide (KI, Sigma-Aldrich, Arklow, Ireland) colorimetric method. A total of 50 µL of PAW or H2O2 standard samples were mixed with 50 µL deionized water and 100 µL 1 M potassium iodide (Sigma-Aldrich, Arklow, Ireland), incubated for twenty minutes, and the absorbance was read at 390 nm wavelength [19 (link)].
Total oxidative species in PAW were measured using the potassium iodide (KI, Sigma-Aldrich, Arklow, Ireland) colorimetric method. A total of 50 µL of PAW or H2O2 standard samples were mixed with 50 µL deionized water and 100 µL 1 M potassium iodide (Sigma-Aldrich, Arklow, Ireland), incubated for twenty minutes, and the absorbance was read at 390 nm wavelength [19 (link)].
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