Isolation and Characterization of CEFFE

CEFFE was provided by SEME Cell Technology Co., Ltd (Shanghai, China), and it was extracted from fresh adipose tissues as previously described (24 (link)). Briefly, the fat tissue was washed with saline several times to discard the blood components. Next, the tissue was emulsified mechanically, centrifuged several times to obtain the lowest fluid layer, and stored at –80°C. The protein concentration of CEFFE was measured using a BCA protein kit (Vazyme, China), and the solution was diluted to 3 mg/mL before use.

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Liu M., Li W., Zhou X., Zhou M., Zhang W., Liu Q., Zhang A, & Xu B. (2022). Cell-Free Fat Extract Improves Ovarian Function and Fertility in Mice With Advanced Age. Frontiers in Endocrinology, 13, 912648.

Publication 2022

BCA protein kit

Blood components Cell Fat tissue Protein Saline Tissue

Corresponding Organization : Shanghai Jiao Tong University

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Variable analysis

independent variables

Extraction method of CEFFE from fresh adipose tissues

dependent variables

Protein concentration of CEFFE

control variables

Washing of fat tissue with saline to discard blood components
Mechanical emulsification of tissue
Centrifugation of tissue to obtain the lowest fluid layer
Storage of CEFFE at -80°C
Dilution of CEFFE to 3 mg/mL before use

controls

Positive control: Not specified
Negative control: Not specified

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