Cell Death Quantification Protocols

For cell death assays^{7 (link),9 (link),11 (link),42 (link)}, cells were trypsinized, collected and stained with trypan blue. The cells were counted with a hemocytometer using the standard protocol, and cells stained blue were considered as dead cells. The cell death was further confirmed by propidium iodide staining followed by FACS analysis. For CellTiter-Glo luminescent assay^{43 (link)}, 10⁴ cells were plated 90 µL per well of a 96-well plate on day 1. The treatments were added to each well of the plates on day 2. The viability of cells was measured using 1:1 dilution of the CellTiter-Glo luminescent reagent (Promega G7572), which was read on a Glomax explorer (Promega) after 10 min of shaking at room temperature. The intensity of luminescence was normalized to that of DMSO control. Percentage of Cell death was counted as 100 minus percentage of cell viability.