CISD2 Knockdown in HCT116 Cells

HCT116 cells were transfected with scramble or CISD2 knockdown lentivirus using six-well plates (NEST Biotechnology) at an MOI of 20, as reported earlier (Shao et al., 2021 (link)). After 24 h of infection, cells were harvested via centrifugation, resuspended in preheated complete culture medium, and administered selective antibiotics. Following a 7-day screening, CISD2 expression was gauged through immunoblotting. Verified cells were then used for subsequent experimentation.

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Xu Y., Tang Q., Ding N., Zhang T, & Luo H. (2023). Ferroptosis-associated gene CISD2 suppresses colon cancer development by regulating tumor immune microenvironment. PeerJ, 11, e15476.

Publication 2023

six-well plates

Antibiotics Cells Centrifugation Culture medium Hct116 cells Infection Lentivirus

Corresponding Organization :

Other organizations : Chenzhou First People's Hospital, Hunan University of Traditional Chinese Medicine

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Variable analysis

independent variables

Transfection of HCT116 cells with scramble or CISD2 knockdown lentivirus

dependent variables

CISD2 expression

control variables

Use of six-well plates (NEST Biotechnology)
MOI of 20 for lentivirus transfection
Harvesting of cells via centrifugation
Resuspension of cells in preheated complete culture medium
Administration of selective antibiotics for a 7-day screening period

controls

Scramble lentivirus as a negative control for CISD2 knockdown

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